FastQCFastQC Report
Mon 6 Jun 2016
ERR1378194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647227
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAACAATCTCGTATGCC11930.18432481957643918Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT1600.039.37168529
CGATAAC1600.039.37168532
AGACCGA1550.039.1902528
GATCGGA356.2509807E-638.565218
CTCGTAT1650.038.17860442
TCTCGTA1650.038.17860441
CGTATGC1750.035.9969744
GCGTTAT250.002107299235.9969737
TCGTATG1750.035.9969743
CCGATAA1700.035.7322931
ACCGATA1700.035.7322930
AAGAGCG1800.035.01874
AGAGCGG1850.034.0696145
ATCTCGT1850.034.0511940
GCGGTTC1950.033.456148
CGGTTCA1950.032.302489
AGCGGTT2050.031.8241337
AATCTCG2000.031.4973539
GAGACCG2000.030.37244627
GAGCGGT2100.030.0137086
Produced by FastQC (version 0.11.5)