FastQCFastQC Report
Mon 6 Jun 2016
ERR1378192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648906
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGTATATCTCGTATGCC10710.16504701759576887Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAA900.042.49810829
CTCGTAT950.040.2644742
AGATCGG800.039.37021317
TATCTCG1000.038.25124739
ATCTCGT1050.036.42975640
TCTCGTA1050.036.42975641
CCGAAGT1050.036.42975631
ACCGAAG1050.036.4269530
GGATCGG250.002107690835.99562519
TCTCGAC250.002107690835.99562520
GATCGGA900.034.99574718
AGACCGA1100.034.7711828
GAGACCG1100.034.768527
CGAAGTA1150.033.2619532
CGTATGC1200.031.8760444
TCGGAAG1000.031.4961720
ATCGGAA1000.031.4961719
TCGTATG1350.028.33425543
GAGATCG1251.8189894E-1225.19693616
CGCTTTT450.00122684824.99696223
Produced by FastQC (version 0.11.5)