FastQCFastQC Report
Mon 6 Jun 2016
ERR1378191.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378191.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146762
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1350.035.0003444
CCGATTG1400.033.75032431
GACCGAT1350.033.33220329
TCGTATG1500.031.50030543
GAGACCG1450.031.03207627
TCTCGTA1550.030.48416541
CGATTGA1650.030.0002932
ACCGATT1500.029.99898330
CGGTTAT300.00514920529.99767514
CGCTACA300.00514920529.99767518
CCGGTAG300.00514920529.99767514
ATCTCGT1750.028.2859940
AGACCGA1600.028.12404428
AGATCGG1004.5474735E-1126.99790817
CTCGTAT1800.026.25025442
TGCTCGG450.001226483725.00024232
GCGGTTA450.001227419524.99697113
GATCGGA1204.5292836E-1022.49825718
ATCGGAA1358.367351E-1121.66498819
AGCGGTT2300.021.5181947
Produced by FastQC (version 0.11.5)