FastQCFastQC Report
Mon 6 Jun 2016
ERR1378190.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378190.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620674
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAAGATATCTCGTATGCC11350.1828657233910233Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1350.040.0017944
TCGTATG1450.037.24304643
GACCGTA1400.036.9629629
GCCCGAG250.002105900636.00161433
CTCGTAT1500.036.0016142
TATCTCG1500.036.0016139
TCTCGTA1500.036.0016141
AGACCGT1500.034.49876428
CCGTAAG1650.032.72873731
AACGACT352.8181518E-432.14429541
GACCGAG352.8208178E-432.13911413
ATCTCGT1700.031.76612940
GCGGTTC1700.031.761018
AGCGGTT1700.031.761017
CGGAAGA1650.031.3776841
GAGCGGT1750.030.8634976
ACCGTAA1750.030.85603730
GAGACCG1700.030.44008827
AGAGCGG1800.030.0061765
GCGGATC300.005144328330.00134335
Produced by FastQC (version 0.11.5)