FastQCFastQC Report
Mon 6 Jun 2016
ERR1378188.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378188.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649533
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCC13970.21507760190783223Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA253.8903792E-544.9971518
CTCGTAT1600.042.1848342
CCGATTA1600.042.1848331
GACCGAT1550.042.09411229
TCGTATG1650.040.906543
ATCTCGT1700.039.7033740
ACCGATT1700.039.7033730
TCTCGTA1700.039.7033741
CGATTAA1750.038.56898532
CGTATGC1850.037.70031444
GAGACCG1850.036.48417327
GAGCGGT1900.035.5377466
GTATGCC1900.035.52406745
AGACCGA1850.035.26803628
AGAGCGG1950.034.6265225
CGGAAGA1900.034.363741
AAGAGCG2050.032.9374244
CGGTTCA2050.032.9247479
GCGGTTC2100.032.1408238
AGCGGTT2100.032.1408237
Produced by FastQC (version 0.11.5)