FastQCFastQC Report
Mon 6 Jun 2016
ERR1378187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936131
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTATGCC15620.1668569890325179Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2400.038.43485341
CGAGTAA2450.037.65046732
ACCGAGT2350.037.33785630
CTCGTAT2500.036.8974642
CGACAAT250.002107621435.9975230
TATCTCG2600.035.47832539
CGTATGC2650.034.80892644
TCGTATG2650.034.80892643
AGATCGG803.6379788E-1233.74587617
GATCGGA803.6379788E-1233.74587618
GACCGAG2700.033.3310429
GAGACCG2750.031.90689527
AGACCGA2750.031.90689528
CGGTTCA3150.030.7105319
GAGCGGT3200.030.2500676
ATCTCGT3050.030.2438240
GCGGTTC3200.030.230688
CGGAAGA3150.030.0219881
TCGGAAG901.2732926E-1129.99633420
CGCTTGC453.4930723E-529.99633416
Produced by FastQC (version 0.11.5)