FastQCFastQC Report
Mon 6 Jun 2016
ERR1378186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7249042
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC2850.016.5770828
CTCGTAT3250.015.92249442
GACCGAA2850.015.78867729
CGTATGC3050.015.49123644
CCGGCTA9500.015.1561916
CGGCTAA9850.014.61764517
ACCCGGC5150.013.97901214
ATCTTCG8300.013.5597075
GAGCGGT3900.013.2746046
CTTCGTA8250.013.0894377
TCGTATA7250.012.7227079
CGCCCGG5850.012.69087913
CGGTTCA3750.012.5985839
CCCGCCA7600.012.4398483
CCCGGCT12050.011.76216215
CGCGATC2901.3551471E-811.63662115
CGCCACC10700.011.5701055
ACCACGC14200.011.56569
AGCGCTC7250.011.4814677
ACCGCGC3551.7462298E-1011.4071679
Produced by FastQC (version 0.11.5)