FastQCFastQC Report
Mon 6 Jun 2016
ERR1378185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057016
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGTGATCTCGTATGCC15700.1485313372739864Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1900.039.07582544
TCTCGTA1950.038.0738841
GCGGTTC2050.037.314098
CCGAGAC2050.037.3140925
GACCGAG2100.035.35431729
CTCGTAT2150.034.53212742
AGACCGA2150.034.53212728
ATGCCGA2300.034.23639322
AAGAGCG2250.034.0133744
AGCGGTT2250.033.9972847
TCGTATG2200.033.74730743
GAGCGGT2350.033.523826
AGAGCGG2300.033.2739525
TGCCGAG2250.032.99736423
ACCGAGT2300.032.2800330
GAGACCG2300.032.2800327
ATCTCGT2350.031.5932240
CGGAAGA2500.030.6265321
AATGCCG2550.029.99760221
CGAGTGT2500.029.69762832
Produced by FastQC (version 0.11.5)