FastQCFastQC Report
Mon 6 Jun 2016
ERR1378184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830925
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCC12300.14802780034299123Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAG207.035169E-444.9960867
TCTCGTA900.037.49899741
CGATCGT250.002107672235.99687230
CGTATGC1000.033.74909644
TCGTATG1000.033.74909643
CTCGTAT1100.030.68099842
CGAGAGT1100.030.68099832
TGCGGGC300.00514863629.9973928
ACGATCG300.00514863629.9973929
CCGAGAG1250.028.79922931
GAGACCG1200.028.12255327
CGGTTCA1400.027.3190549
ACCGAGA1250.026.99765230
AGACCGA1250.026.99765228
AGAGCGG1350.026.6755855
GACCGAG1300.025.95928229
ATCTCGT1350.024.9993340
CCGAGAC1350.024.9978325
GCGGTTC1400.024.1050478
GAGCGGT1600.022.5075246
Produced by FastQC (version 0.11.5)