FastQCFastQC Report
Mon 6 Jun 2016
ERR1378183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658387
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGTAATCTCGTATGCC13610.20671732582812236Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2300.042.06891341
CGTATGC2200.041.935544
AGACCGG2150.040.81443828
ATCTCGT2400.040.3160440
CGGCAGT2300.040.1122232
ACCGGCA2300.040.10612530
GAGACCG2200.039.88683727
CCGGCAG2350.039.25876631
GACCGGC2250.039.0004629
CTCGTAT2500.038.703442
GAGCGGT2450.036.74356
AGCGGTC250.002106333636.00042326
CCGAGAC2450.035.8167525
AGAGCGG2550.035.302585
AAGAGCG2750.035.1902544
TCGTATG2650.034.8117343
GCGGTTC2600.034.6079068
AATCTCG2700.034.16966639
CGGTTCA2650.033.9549269
AGCGGTT2700.033.3261347
Produced by FastQC (version 0.11.5)