FastQCFastQC Report
Mon 6 Jun 2016
ERR1378181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152602
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT2000.040.49703229
CGTATGC2150.038.71809444
CTCGTAT2200.037.83813542
TCTCGTA2250.035.9973641
TCGTATG2400.034.6849643
CCGGTGA2450.033.97710431
CGGTGAT2600.032.88220632
GCGGTTC2550.032.6446658
GAGACCG2500.032.3976327
CGGTTCA2650.032.2617879
ACCGGTG2600.032.01688430
AGACCGG2550.031.76237728
ATCTCGT2600.031.15156440
GTATGCC2600.031.15156445
CGGAAGA2800.030.5573391
AGAGCGG2750.030.2836485
GAGCGGT2800.029.7428676
AGCGGTT2900.028.7047927
CCGAGAC3000.027.74796725
GATCGGA755.6432327E-826.99802218
Produced by FastQC (version 0.11.5)