FastQCFastQC Report
Mon 6 Jun 2016
ERR1378180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739168
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACATGATCTCGTATGCC14660.19833109658426773Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC1900.043.8157930
TCGTATG2000.042.74421743
CGTATGC2050.041.70449444
GACCGGA2000.041.62529
TCTCGTA2150.039.7647541
AGACCGG2100.039.64285328
CGGACAT2150.038.72093232
CCGGACA2150.038.72093231
CTCGTAT2250.037.9974342
CGGAAGA2200.037.8716551
GCGGTTC2350.037.3353738
GAGCGGT2300.037.1864936
AGCGGTT2350.036.3780567
CGGTTCA2500.035.995139
GAGACCG2400.035.62018227
TGCCGAG2500.035.09762623
GTATGCC2550.034.4094445
AGAGCGG2500.034.2115755
ATCTCGT2500.034.240
CCGAGAC2600.033.7477225
Produced by FastQC (version 0.11.5)