FastQCFastQC Report
Mon 6 Jun 2016
ERR1378179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477404
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCC13880.2907390805271845Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1500.042.00303341
GACCGGT1500.041.9986329
AGACCGG1500.041.9986328
CGTATGC1600.039.37784244
CTCGTAT1600.039.37784242
ACCGGTG1600.039.3737230
GAGACCG1600.039.3737227
CCGGTGT1600.039.3737231
TCGTATG1700.037.05761743
AGATCGG504.8747097E-835.99505617
GATCGGA504.8747097E-835.99505618
GTATGCC1750.034.7167945
ATCTCGT1850.034.0600840
AATCTCG1900.033.1637639
CGGTGTG1900.033.15681532
GAGCGGT2150.031.4041966
GCGGTTC2150.031.387758
AAGAGCG2200.030.6904644
AGCGGTT2200.030.6743937
ATCGCGC300.005137196730.0084535
Produced by FastQC (version 0.11.5)