FastQCFastQC Report
Mon 6 Jun 2016
ERR1378178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795271
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTGAATCTCGTATGCC46370.5830716824830781Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8110.10197781636699943Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5250.038.99907744
ATCTCGT5250.038.57051540
GAGACCG5300.038.20423527
TCGTATG5450.037.98074743
CCGCAGT5400.037.91576431
TCTCGTA5350.037.84956741
GACCGCA5400.037.4967529
CTCGTAT5500.037.22638742
AGACCGC5450.037.15274428
AATCTCG5600.036.1598639
ACCGCAG5750.035.6056130
AGCGGTT6250.034.914787
GCGGTTC6150.034.75098
AAGAGCG6100.034.688764
CGGAAGA6000.034.529581
CGCAGTG6100.033.9336232
GTATGCC6150.033.65773845
GAGCGGT6350.033.320966
AGAGCGG6350.033.320965
GATCGGA754.7293724E-1132.99506818
Produced by FastQC (version 0.11.5)