FastQCFastQC Report
Mon 6 Jun 2016
ERR1378177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905305
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCTGAATCTCGTATGCC26260.29006798813659485Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA3200.043.5958941
CTCGTAT3300.042.27480342
CCGAGCT3450.039.78236431
CGAGCTG3450.039.78236432
AAGAGCG3550.039.306394
CGTATGC3550.039.29553244
TCGTATG3600.038.74976343
GCGGTTC3600.038.7454838
CGGAAGA3700.038.3338661
ATCTCGT3650.038.22105440
ACCGAGC3600.038.12265830
AGAGCGG3700.037.7128875
AATCTCG3750.037.20182839
GAGACCG3700.037.0923227
CGGTTCA3850.036.813899
GACCGAG3750.036.59977729
GAGCGGT3850.036.2435536
AATGCCG4000.034.87093421
AGACCGA3950.034.7466228
CCGAGAC4050.034.4423325
Produced by FastQC (version 0.11.5)