FastQCFastQC Report
Mon 6 Jun 2016
ERR1378175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880648
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCC12410.1409189596751483Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1600.039.3720744
GATCGAA1600.039.3720734
TCGAATC1600.039.3720736
ACCGGAT1550.039.1906330
CGGATCG1650.038.17897432
CGAATCT1750.037.2829437
CTCGTAT1700.037.05606542
GACCGGA1650.036.8154429
GGATCGA1750.035.99732233
TCGTATG1750.035.99732243
CCGGATC1700.035.7326331
ATCTCGT1800.034.99739540
GCGGTTC1900.034.3395468
CGGTTCA1950.033.459059
TCTCGTA1900.033.15542641
AGACCGG1850.032.83539228
AGCGGTT1950.032.3052867
GAGACCG1900.031.97130427
GAGCGGT2050.031.8431726
CGGAAGA2200.031.7274061
Produced by FastQC (version 0.11.5)