FastQCFastQC Report
Mon 6 Jun 2016
ERR1378174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162879
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTACGAATCTCGTATGCC12890.11084558238647357Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG1900.043.81281732
CTCGTAT2050.040.60700242
CCGGTAC2050.040.60700231
ACGAATC2000.040.49725336
TCGTATG2150.039.76474443
GGTACGA2100.039.64016733
ACCGGTA2200.038.86099630
CGTATGC2350.038.29527744
TCTCGTA2200.037.8383441
GACCGGT2150.037.67186429
TACGAAT2250.036.9974935
GTACGAA2250.036.9974934
CGAATCT2250.035.9975637
AGACCGG2300.035.21528
GAGACCG2350.034.46574827
AGATCGG1000.033.7477117
GATCGGA1000.033.7477118
TCGGAAG1000.033.7477120
ATCTCGT2600.031.15173340
AATCTCG2650.030.56396539
Produced by FastQC (version 0.11.5)