FastQCFastQC Report
Mon 6 Jun 2016
ERR1378173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199920
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG1650.038.1811532
GAGACGT1700.037.0581733
TCGTATG1700.037.05662543
CGTATGC1800.036.2493644
GACGTAT1800.034.99938235
TCTCGTA1800.034.99938241
CTCGTAT1900.034.34150342
GACCGAG1900.034.34007329
GAGACCG2000.033.74827
AGACGTA2050.031.82870934
AGATCGG950.030.78636717
ACCGAGA2150.030.34703830
AGACCGA2150.030.34703828
ATCTCGT2100.029.99947440
GAGCGCC300.005148838729.99822225
GATCGGA1001.8189894E-1229.24704718
TATCTCG2250.027.99950639
CGTATCT2500.026.09954337
ACGTATC2450.025.71383336
AGAGCGG2500.025.2090135
Produced by FastQC (version 0.11.5)