FastQCFastQC Report
Mon 6 Jun 2016
ERR1378172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810162
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCC10680.13182548675450095Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1250.043.19632744
TCGTATG1350.041.6631243
CATCTCG1350.039.9990739
TCTCGTA1350.039.99659741
ATCTCGT1450.037.2405140
CTCGTAT1550.036.2872442
CGCCAAA250.002106983435.9991638
AGATCGG1050.034.282817
ACCGAGT1650.034.0880130
CTTCGTA606.4319465E-933.747137
GACCGAG1700.033.08542329
AGACCGA1650.032.7244928
CGTATAA501.98198E-631.49732210
GATCGGA1200.029.9974518
GAGACCG1800.029.9974527
GTATGCC1850.029.18670745
TTCGTAT702.8863724E-828.926118
TCGTATA554.1656986E-628.6339289
CGAGTAG1900.028.42039132
ATCGGAA1300.027.68995319
Produced by FastQC (version 0.11.5)