FastQCFastQC Report
Mon 6 Jun 2016
ERR1378171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050706
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1350.034.99859244
ATCTCGT1650.029.99879340
CTCGTAT1600.029.53006442
TCGTATG1600.029.53006443
CGGAAGA1550.029.0532151
CGGCTAG1650.028.6352132
GACCGGC1650.028.6338529
ACCGGCT1700.027.7916830
GCGGTTC1800.027.4962778
CGGTTCA1800.027.4962779
TCTCGTA1750.026.99891541
ATCGGAA507.1644215E-526.99634719
AGCGGTT1900.026.0491057
GAGACCG1950.025.38238527
GAGCGGT2000.024.7631476
CCGAGAC2000.024.74782825
ATGCCGA2200.023.51954322
GTATGCC2050.023.04785545
AAGAGCG2150.023.0354864
GATCGGA602.4695744E-422.49695418
Produced by FastQC (version 0.11.5)