FastQCFastQC Report
Mon 6 Jun 2016
ERR1378170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649129
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCC14400.22183572140514443Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1600.036.56137542
GACCGCA1650.035.45345329
TCTCGTA1650.035.45345341
AGACCGC1650.035.45345328
GATCGGA458.699499E-734.99622718
ATCTCGT1700.034.41070640
CCGCAGA1900.034.3410531
TATCTCG1650.034.0898639
TCGTATG1800.033.74896243
CGCAGAG1900.033.15687632
CGTATGC1900.031.972744
GAGCGGT2000.030.3834276
GCGGTTC2000.030.3717258
GAGACCG1950.029.99907527
CGGTTCA2050.029.6309499
AGCGGTT2050.029.6309497
ACCGCAG2150.029.30142230
CCGAGAC2050.028.53570625
GTATGCC2350.026.80768445
AGATCGG608.191224E-626.24717117
Produced by FastQC (version 0.11.5)