FastQCFastQC Report
Mon 6 Jun 2016
ERR1378169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358258
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCC17760.13075571798583185Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2950.038.90024644
CTCGTAT2900.038.79361342
TCGTATG3050.038.3611643
GACCGTG3050.036.8857329
GATCGGA1100.036.814618
CCGTGCA3150.035.71475231
TCTCGTA3300.034.77318241
AGACCGT3300.033.40952728
ATCTCGT3600.031.87541840
AATCTCG3600.031.87541839
AGATCGG1350.031.66358617
GAGACCG3600.030.625427
CGTGCAG3900.028.8465332
AGCGGTT3900.028.8433447
GTATGCC3950.028.48243545
GAGCGGT4000.028.1274386
ATCGGAA1450.027.92831619
GCGGTTC4100.027.4363548
AGAGCGG4150.027.1107855
ACCGTGC4250.027.00035530
Produced by FastQC (version 0.11.5)