FastQCFastQC Report
Mon 6 Jun 2016
ERR1378168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962583
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCC12130.1260151072686719Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1150.039.12808642
ACCGCTC1150.037.17168430
GACCGCT1100.036.81597529
GAGACCG1150.035.2152827
CCGCTCT1250.034.1979531
AGACCGC1200.033.74797428
CGTATGC1300.032.8826444
CGCTCTC1350.031.66476632
TATCTCG1350.031.66476639
CGCTAGG300.005148358629.99819834
ATCTCGT1550.029.03051640
TCTCGTA1600.028.12331241
GCGGTTC1600.026.7171468
AGCGGTT1650.025.9075367
GTATGCC1750.025.71274245
CCGAGAC1600.025.3109825
GAGCGGT1700.025.1546966
AGATCGG551.3685343E-424.54398217
TCGTATG1750.024.42710543
AGAGCGG1800.023.7572125
Produced by FastQC (version 0.11.5)