FastQCFastQC Report
Mon 6 Jun 2016
ERR1378167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182810
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGCAATCTCGTATGCC13540.11447316136995798Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1950.036.92075344
TCTCGTA1950.036.92075341
CTCGTAT2000.035.99773442
ACCGAGT2050.035.1197430
TCGTATG2050.035.1197443
AGACCGA2150.032.4398228
GAGACCG2250.031.99798627
GACCGAG2200.031.70254929
GATCGGA1000.031.49535418
TAGACGA501.9839663E-631.49535421
ATCTCGT2350.030.63637240
AGATCGG1050.029.99557717
CGAGTGC2300.029.3459832
GCGGTTC2650.027.1658028
CCGAGAC2800.026.51618825
CCGAGTG2600.025.95990431
GAGCGGT2700.025.8470066
ATCGGAA1251.8189894E-1225.19628519
AATCTCG2950.025.16790639
CGGTTCA3000.023.9964629
Produced by FastQC (version 0.11.5)