FastQCFastQC Report
Mon 6 Jun 2016
ERR1378166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153924
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCC16150.13995722421927267Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG2100.040.7130331
TCTCGTA2100.040.71126641
TGCGAAA301.14071314E-437.49559421
CGGTAGC2350.036.3818632
CGTATGC2350.036.3802844
CTCGTAT2350.036.3802842
GACCGGT2250.035.9957729
ACCGGTA2400.035.62081530
AGACCGG2350.034.46403528
ATCTCGT2600.032.88360240
TCGTATG2750.032.7234343
GATCGGA1700.031.75959618
AATCTCG2800.031.33832239
ATCGGAA1750.030.85218219
AGATCGG1800.029.99517417
ATTACCG300.005151315629.99517411
GAGACCG2850.029.20709627
TCGGAAG2050.026.3383720
GTATGCC3500.025.7123845
GCGGTTC3600.024.995988
Produced by FastQC (version 0.11.5)