FastQCFastQC Report
Mon 6 Jun 2016
ERR1378165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965970
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCC12580.1302317877366792Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCG1800.036.2472533
CGATAGC1850.035.26759332
TAGCGAT2000.034.87235335
ATAGCGA1950.034.6127634
CCGATAG1850.034.05146831
ACCGATA1850.034.05146830
GACCGAT1800.033.7474429
AGATCGG1350.033.33080317
TCGGAAG1400.032.1404220
ATCGGAA1400.032.1404219
CGTATGC2100.032.14041544
GATCGGA1450.031.03212718
TCTCGTA2200.030.67948741
CTCGTAT2250.029.99772342
AGACCGA2100.028.92637428
ATCTCGT2450.028.46722440
TCGTATG2450.027.54892743
GAGACCG2400.027.18543627
AGCGATC2600.026.82488436
CGCGATC608.194609E-626.24800715
Produced by FastQC (version 0.11.5)