FastQCFastQC Report
Mon 6 Jun 2016
ERR1378164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058139
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGACGATCTCGTATGCC11880.11227258422570191Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAG1300.039.80484830
GTAGACG1400.038.57049633
GACCGTA1400.036.96164729
CGTATGC1600.036.5616244
TAGACGA1500.035.99913434
TCTCGTA1650.035.45536441
CGTAGAC1550.034.8378732
CCGTAGA1500.034.4991731
ATCTCGT1700.034.41256340
CTCGTAT1700.034.41256342
GACGATC1750.033.42934436
AGACCGT1550.033.38471628
GAGACCG1750.032.14056427
AGACGAT1900.030.7887335
TCGTATG2000.029.24929443
AGATCGG852.0372681E-1029.11556817
GATCGGA852.0372681E-1029.11556818
GCGGTTC2100.026.7838028
ACGATCT2250.026.000637
GTATGCC2250.025.9993745
Produced by FastQC (version 0.11.5)