FastQCFastQC Report
Mon 6 Jun 2016
ERR1378163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153933
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTCG207.0312613E-445.00264734
CGTATGC1000.040.50413544
AGATCGG751.8189894E-1235.99431617
CTCGTAT1250.030.6044542
GACCGCA1250.030.60179729
CGCAGAC1250.030.60179732
AGACCGC1250.028.80169128
TCGTATG1350.028.33376943
ATCTCGT1450.027.93509940
TCTCGTA1400.027.32540341
GATCGGA1004.5474735E-1126.99573718
CCGCAGA1350.026.66823431
ACCGCAG1400.025.71579730
GAGACCG1500.025.49928927
ATCGGAA1152.6557245E-1023.47455219
CGGTTCA1650.023.1781589
GCGGTTC1700.022.4964478
AGAGCGG1750.021.8593755
GGACGTT654.2340858E-420.77225136
TCGGAAG1351.9663275E-919.99770720
Produced by FastQC (version 0.11.5)