FastQCFastQC Report
Mon 6 Jun 2016
ERR1378162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477921
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGTAGATCTCGTATGCC11540.2414625011246629Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA1100.044.9970732
CCGACGT1050.044.9970731
ACCGACG1050.044.99236330
GATCGGA253.8912724E-544.9923618
CGTAGAT1150.043.04517735
AGACCGA1100.042.94725428
TCGTATG1250.041.3929743
CTCGTAT1200.041.2516342
TCTCGTA1200.041.2516341
GACGTAG1200.041.24731433
ATCTCGT1250.039.6057140
GACCGAC1200.039.36831329
CGTATGC1400.036.96574844
GTAGATC1350.036.67195536
GAGCGGT1400.035.3696446
ACGTAGA1400.035.3585434
AGCGGTT1450.034.1321377
GCGGTTC1400.033.7442748
GAGACCG1400.033.74427427
GTATGCC1500.033.00130545
Produced by FastQC (version 0.11.5)