FastQCFastQC Report
Mon 6 Jun 2016
ERR1378161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671002
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAGTACATCTCGTATGCC16980.25305438731926283Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA2350.042.12312729
TCTCGTA2500.041.3986341
CGGAGTA2600.039.80637432
ACCGGAG2600.039.8034130
CCGGAGT2700.039.1653731
GATCGGA356.249016E-638.5672818
CGTATGC2700.038.33206644
GAGACCG2700.037.49596427
ATCTCGT2800.036.96581640
AGACCGG2750.035.99612428
CGGAAGA2950.035.8730051
TCGTATG2900.035.68847343
GCGGTTC2900.035.6858148
CTCGTAT2950.035.08358442
AGAGCGG3000.034.5091445
AGCGGTT3000.034.4962847
GTATGCC3050.033.933345
CCGAGAC3000.033.74888225
AGATCGG401.560752E-533.7463717
ATCGGAA401.560752E-533.7463719
Produced by FastQC (version 0.11.5)