FastQCFastQC Report
Mon 6 Jun 2016
ERR1378160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176566
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTAGATCTCGTATGCC17520.14890792356739868Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC2400.039.3707628
CGCAGTA2600.038.07767532
GACCGCA2500.037.79593329
CGTATGC2700.035.00069444
TCTCGTA2700.035.00069441
CCGCAGT2700.035.00069431
GAGACCG2700.034.99623527
ATCTCGT2750.034.3643240
CTCGTAT2800.033.75066842
ACCGCAG2850.033.15432730
TCGTATG3050.032.4582843
GATCGGA801.0004442E-1030.9341718
GCGGTTC3150.029.9967738
CCGGCGT300.005150017329.9967716
CGGAAGA3150.029.301241
GAGCGGT3350.028.8909936
TCGGAAG952.3646862E-1128.41799420
AGCGGTT3400.027.7911247
CGGTTCA3450.027.3883559
AGAGCGG3650.026.516395
Produced by FastQC (version 0.11.5)