FastQCFastQC Report
Mon 6 Jun 2016
ERR1378159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110023
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCATAGATCTCGTATGCC13030.11738495508651624Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1050.040.7112718
AGATCGG1100.038.86075617
CGGCATA1700.035.73425732
AGACCGG1600.035.1536528
CCGGCAT1700.034.41076731
ACCGGCA1700.034.40921430
TCGGAAG1350.033.33086420
TCTCGTA1700.033.08727341
GACCGGC1750.032.14047629
CGTATGC1900.030.7885844
ATCTCGT1900.029.60440440
TCGTATG2000.029.24915143
ATCGGAA1550.029.03010719
CTCGTAT1950.028.84531642
ACGCCCG406.1686814E-428.12291712
GAGACCG1950.027.69025627
CGAGACC2400.024.37319626
GTATGCC2450.023.87685845
GATCTCG2450.023.87685839
GCGGTTC2400.023.4357648
Produced by FastQC (version 0.11.5)