FastQCFastQC Report
Mon 6 Jun 2016
ERR1378158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149415
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTGAGATCTCGTATGCC15870.13807023572860977Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCG2000.034.8735827
TCTCGTA1950.034.61397641
ATCTCGT2050.034.02300640
AGACCGT2000.033.74862728
CGTGTGA2150.032.44054432
CGTATGC2100.032.14155244
CTCGTAT2200.030.68057342
GATCGGA1700.030.43729418
GACCGTG2350.029.67964629
AGATCGG1700.029.11393417
TCGTATG2400.029.0613243
AATCCGT406.167608E-428.12385736
ATCGGAA1800.027.49649219
CCGTGTG2500.026.99890331
CGAGACC2700.024.99898326
GTATGCC2750.024.54445645
ACCGTGT3000.022.49908630
GATCTCG3300.022.49908639
CGAGATC2450.022.03800415
GAGCGGT3150.021.4370296
Produced by FastQC (version 0.11.5)