FastQCFastQC Report
Mon 6 Jun 2016
ERR1378157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239149
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1550.037.74037642
TCTCGTA1650.035.45308341
TCGTATG1650.035.45308343
CGTATGC1700.034.41034344
GACCGAG1800.032.49865729
ACCGAGT1800.032.49865730
ATCTCGT2050.031.82795340
CGAGTGA1850.031.62031732
GAGACCG2100.028.9262127
CCGAGTG2200.027.61249731
CATCTCG2350.026.80740539
CGGTTCA2200.026.588749
AGCGGTT2200.026.588747
GCGGTTC2250.025.997888
ATCGGAA709.315154E-725.71218919
AGACCGA2350.024.89259128
AATGCCG2600.024.2287921
GTATGCC2500.023.39903545
CCGAGAC2600.023.36347825
GAGCGGT2550.022.949496
Produced by FastQC (version 0.11.5)