FastQCFastQC Report
Mon 6 Jun 2016
ERR1378156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633538
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATGAGATCTCGTATGCC11990.18925463034577247Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1450.037.23909444
CCGCATG1400.036.96493531
CTCGTAT1400.036.96201742
GACCGCA1400.036.96201729
TCTCGTA1400.036.96201741
CGCATGA1500.036.0006332
ATCTCGT1500.034.50060340
AGACCGC1500.034.4978828
TCGTATG1600.033.74526643
ACCGCAT1550.033.38504430
ATCGGAA708.1308826E-1032.13834819
GTATGCC1800.029.99815845
AGATCGG751.7025741E-929.9957917
GAGACCG1750.029.5672827
GATCGGA803.3942342E-928.12105418
CCGAGAC1900.027.23516825
CGGTTCA2100.026.7819569
GCGGTTC2000.025.8713688
TCGGAAG901.1896191E-824.99846520
AAGAGCG2100.024.6530194
Produced by FastQC (version 0.11.5)