FastQCFastQC Report
Mon 6 Jun 2016
ERR1378153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937173
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCAGATCTCGTATGCC9680.10328936066233237Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1200.037.50089341
GACCGTA1150.035.2144729
AGACCGT1150.035.2144728
AGATCGG401.5611471E-533.747217
GATCGGA401.5611471E-533.747218
CGTAGCA1450.032.58524332
CTCGTAT1400.032.1436242
CCGTAGC1400.032.14190331
GCGATTA300.00514618230.00071335
CGTATGC1450.029.48345844
TCGTATG1450.029.48031243
GAGACCG1400.028.9261727
ACCGTAG1500.028.49763530
ATCTCGT1550.027.58277140
GAGCGGT1650.027.2762856
GTATGCC1750.024.42915245
AGAGCGG1850.024.3274975
CCGAGAC1700.023.82155225
CGGTTCA1800.023.7480289
AGCGGTT1800.023.7480287
Produced by FastQC (version 0.11.5)