FastQCFastQC Report
Mon 6 Jun 2016
ERR1378150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650791
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTGTAATCTCGTATGCC12640.1942251813562265Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7460.11462973519916532Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1200.039.3758142
GACCGTC1200.039.3727829
AGATCGG1200.039.36975517
GATCGGA1200.039.36975518
CGTATGC1300.038.077744
TCTCGTA1250.037.80077741
AGACCGT1350.036.664628
ATCTCGT1300.036.34689740
TCGTATG1400.035.3551543
ACCGTCT1400.035.3551530
GAGACCG1350.034.99802827
ATCGGAA1400.033.74550619
TCGGAAG1350.033.32889620
CCGTCTG1600.030.93813131
CGTCTGT1650.030.00061632
GGCTACG300.005147055329.99830843
GTATGCC1900.028.42163545
GAGATCG1800.026.24650616
CGAGATC1850.025.5371415
AATCTCG2000.024.75050539
Produced by FastQC (version 0.11.5)