FastQCFastQC Report
Mon 6 Jun 2016
ERR1378149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448801
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTCTAATCTCGTATGCC15910.10981494352916651Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2650.037.355844
CATGTCG250.002108392335.99616214
CTCGTAT2800.035.3545942
TCTCGTA2800.035.3545941
GACCGTG2950.032.79424729
AGACCGT2950.032.79424728
TCGTATG3200.030.93526843
GAGACCG3150.030.71101227
ATCTCGT3300.030.68066640
GCGGTTC3250.030.4582928
CCGTGTC3400.029.11655231
CGGTTCA3400.029.1145449
GAGCGGT3500.028.2963716
AGAGCGG3500.028.2963715
AGCGGTT3550.027.884357
CGGAAGA3750.027.6237531
CCGAGAC3650.026.5049425
AAGAGCG3850.025.7239724
ACCGTGT3950.025.06148330
AATCTCG4050.024.99905839
Produced by FastQC (version 0.11.5)