FastQCFastQC Report
Mon 6 Jun 2016
ERR1378148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070934
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCC12360.11541327476763273Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC1600.039.3732431
GACCGAT1500.038.9964429
TCGTATG1750.037.2823143
ACCGATA1700.037.0554430
CTCGTAT1750.035.99839442
TCTCGTA1750.035.99839441
CGATACT1750.035.99839432
CGTATGC1800.034.99843644
ATCTCGT1950.032.3062540
AGACCGA1900.030.78666128
GCGGTTC2000.030.370818
AGCGGTT2100.028.9245827
CATCTCG2300.028.368339
GAGACCG2150.027.2055527
CGGTTCA2300.026.4093999
TCGGAAG901.19252945E-824.99654820
CGGAAGA2450.024.8203091
GAGCGGT2450.024.8098726
AAGAGCG2550.023.8369374
AGAGCGG2600.023.3785325
Produced by FastQC (version 0.11.5)