FastQCFastQC Report
Mon 6 Jun 2016
ERR1378146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855791
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTATGATCTCGTATGCC10830.12654958979470454Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCCG250.002103654436.010995
ATCGGAA250.002107882635.9962619
CGTATGC1000.033.74846644
GCGGTTC1000.033.7464948
GACCGTC1050.032.14139629
AGACCGT1050.032.14139628
CCGTCTA1000.031.4985731
CGTCTAT1050.029.99863632
AGATCGG300.005149146529.99688317
GATCGGA300.005149146529.99688318
TCGGAAG300.005149146529.99688320
ACCGTCT1100.028.63506330
GAGACCG1200.028.12372227
CGTTATG406.1685924E-428.12207811
CGCCTGG654.8753645E-727.6894313
TCTCGTA1150.027.3900641
AGCGGTT1250.026.9971987
GAGCGGT1300.025.9694616
ATCTCGT1300.025.9603640
GCCCGAG450.001219952625.0222571
Produced by FastQC (version 0.11.5)