FastQCFastQC Report
Mon 6 Jun 2016
ERR1378145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068282
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTAGTATCTCGTATGCC15660.14659050700096043Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2700.035.83143242
ATCTCGT2750.035.1799540
CGTCTAG2750.035.1799532
GACCGTC2750.035.17830729
GATCGGA653.6743586E-1034.6119318
CCGTCTA2800.034.5517431
TCTCGTA2800.034.5517441
TCGTATG3000.033.7482143
GAGACCG2900.033.35873827
ACCGTCT2900.033.35873830
AGACCGT3000.032.99670828
GCGGTTC3000.032.246788
AGCGGTT3100.031.9322957
AGAGCGG3050.031.735975
GAGCGGT3100.031.2240986
CGGTTCA3100.031.206569
TATCTCG3200.030.2327739
CGTATGC3250.029.76764944
CGGAAGA3250.029.0998971
AGATCGG803.3978722E-928.12219217
Produced by FastQC (version 0.11.5)