FastQCFastQC Report
Mon 6 Jun 2016
ERR1378144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978176
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGTCTATCTCGTATGCC13610.13913651531012824Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1700.039.70430441
GACCGTA1700.039.70227429
CGTAGTC1750.038.56989332
CGTATGC1800.037.4985144
CTCGTAT1800.037.4985142
AGACCGT1800.037.4965928
CCGTAGT1850.036.48503531
ACCGTAG1900.035.52308730
GAGACCG1850.035.26706327
TCGTATG2050.032.92551843
ATCTCGT2150.032.4405740
TATCTCG2100.032.1415839
GAGCGGT2100.031.0813166
AGAGCGG2150.030.3584965
GCGGTTC2350.029.6781548
CGGAAGA2300.028.3945641
CGAGACC2450.027.54851726
GATCGGA507.161757E-526.99754718
CGGTTCA2700.026.6642429
AGCGGTT2550.026.4681847
Produced by FastQC (version 0.11.5)