FastQCFastQC Report
Mon 6 Jun 2016
ERR1378143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915413
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCC12000.1310883721336708Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1800.038.7477441
CGTATGC1850.037.7005144
TATCTCG1850.037.7005139
CCGGTAT1850.037.7005131
CTCGTAT1900.036.7083942
GACCGGT1950.035.76714729
CGGTATC1950.035.76714732
AGACCGG2000.034.87296728
TCGTATG2050.034.02240843
ATCTCGT2200.031.70269840
ACCGGTA2200.031.70269830
GATCGGA950.030.7876818
AGACGAT300.005148183629.99825136
GTATGCC2350.028.7217345
GAGACCG2450.028.4677327
AGAGCGG2650.028.0264235
ATCGGAA1150.027.3897119
GCGGTTC2750.026.9984288
AGATCGG1105.456968E-1226.5893617
AAGAGCG3000.026.2570744
Produced by FastQC (version 0.11.5)