FastQCFastQC Report
Mon 6 Jun 2016
ERR1378142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179031
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATCAATCTCGTATGCC13930.11814786888555093Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC1250.035.99860832
GATCGGA1250.035.9970818
AGATCGG1200.035.62211217
GACCGCT1400.032.14161729
CCGCTAT1400.032.14161731
TCGGAAG1450.031.03196720
ATCGGAA1450.031.03196719
ATCTCGT1500.029.9988440
CTCGTAT1500.029.9988442
TCGTATG1500.029.9988443
ACCGCTA1550.029.03113730
AGACCGC1550.029.03113728
CGTATGC1600.028.12391344
TCTCGTA1600.028.12391341
GAGACCG1850.025.53847127
ACATCCG500.002260133222.507725
TCGTTCC550.00392781620.4624333
AATCTCG2200.020.45375439
GCGGTTC2200.020.4528878
GAGCGGT2250.020.0068636
Produced by FastQC (version 0.11.5)