FastQCFastQC Report
Mon 6 Jun 2016
ERR1378141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986771
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGATCTATCTCGTATGCC11340.11492028038926966Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1450.038.79205341
CTCGTAT1550.034.8377742
TCGTATG1550.034.8377743
ATCTCGT1500.034.4990740
AGAGCGG1600.033.7610635
TATCTCG1550.033.38619639
AGACCGT1550.033.38450228
GAGCGGT1650.032.7386
CCGTGAT1700.031.76384731
ACCGTGA1700.031.7622430
CGTATGC1750.030.85630844
GCGGTTC1750.030.8547448
AGCGGTT1750.030.8547447
GAGACCG1700.030.43881227
GTCCGGG300.005147590329.99919145
CGGTTCA1800.029.997679
CGTTAAG300.005148865729.9976727
GACCGTG1800.028.74776629
GTATGCC1950.027.69155945
CGGAAGA2100.026.8012891
Produced by FastQC (version 0.11.5)