FastQCFastQC Report
Mon 6 Jun 2016
ERR1378140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417773
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC1700.034.41114429
CTCGTAT1850.034.0534442
ACCGTCA1900.033.157330
CGTATGC1950.032.30711444
TCTCGTA2050.031.82869541
TCGTATG2100.031.06977343
CCGTCAT2100.029.9994631
ATCTCGT2250.028.99947540
GATCGGA702.8896466E-828.9260118
AGAGCGG2500.027.0052285
AGCGGTT2450.026.6302957
AGACCGT2200.026.5904328
CGTCATC2400.026.24952732
GCGGTTC2400.026.2476758
GAGCGGT2600.025.1010136
AAGAGCG2950.023.6486454
CGGAAGA2800.023.3187791
CGGTTCA2750.022.9070639
CTGTCGG500.002266034322.49800913
GTCGGCT702.7054184E-522.49800915
Produced by FastQC (version 0.11.5)