FastQCFastQC Report
Mon 6 Jun 2016
ERR1378138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007036
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTA1400.036.96240631
GCGCTGA250.002107509935.99817339
CGACTAC1450.035.68784332
GACCGAC1500.034.49653629
ACCGACT1500.034.49653630
CGTATGC1550.033.385444
TCTCGTA1550.033.385441
ATCTCGT1600.032.34210640
TATCTCG1650.031.36204539
AGACCGA1650.031.36048728
TCGTATG1750.030.85557443
CTCGTAT1700.030.43963242
CGGATAC300.00513450130.0148724
GAGACCG1900.027.23410827
GATCGGA755.6432327E-826.99728818
AGATCGG856.486516E-926.46793217
TTTCGCC709.265477E-725.7270326
CCGAGAC1950.025.38206725
ATCGGAA801.0545409E-725.30995819
AAGAGCG2050.025.2564164
Produced by FastQC (version 0.11.5)