FastQCFastQC Report
Mon 6 Jun 2016
ERR1378137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312885
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC950.040.25988844
CGCATAC1000.035.99707432
GACCGCA1150.031.30180229
AGACCGC1200.029.9975628
CCGCATA1200.029.9975631
TCTCGTA1300.029.42068741
TATCTCG1350.028.33103439
TCGTATG1400.027.31920843
GCGGTTC1450.026.3761628
ATCTCGT1500.025.49792940
CTCGTAT1500.025.49792942
ACCGCAT1450.024.8255730
AGCGGTT1550.024.6744757
GAGCGGT1800.023.7625526
CGGTTCA1650.023.1790529
CCTTCGT1750.021.8770311
GAGACCG1750.021.85536827
CCGCCAC955.5028977E-721.3278664
ACGGGAT1700.021.17394313
AATGCCG1850.020.67320821
Produced by FastQC (version 0.11.5)