FastQCFastQC Report
Mon 6 Jun 2016
ERR1378136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227854
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGTATATCTCGTATGCC18800.1531126664896641Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1750.043.71532
CCGTCGT1850.040.13578831
GCGGTTC2000.039.3724338
ACCGTCG1900.039.076430
GTCGTAT2000.038.2506233
GACCGTC2000.037.1225829
AGCGGTT2250.036.997597
TATCTCG2050.036.22010439
AGACCGT2200.033.747828
CGTATGC2300.033.26005644
GAGCGGT2500.032.4044886
CGTATAT2300.032.28313435
TCGTATG2300.032.2818243
AGAGCGG2450.032.147315
TCTCGTA2350.031.59497341
GAGACCG2350.031.59368527
ATCTCGT2400.030.93800740
CTCGTAT2400.030.93674542
CGGTTCA2550.030.8803399
ATGCCGA2500.030.59925322
Produced by FastQC (version 0.11.5)