FastQCFastQC Report
Mon 6 Jun 2016
ERR1378135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292414
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTACATCTCGTATGCC13280.10275345206721684Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1500.037.4988444
GACCGTA1500.037.4988429
TCTCGTA1650.034.0898541
CGTACTA1650.034.0898532
CTCGTAT1700.033.0872142
CCGTACT1700.033.0872131
ACCGTAC1700.033.0872130
ATCTCGT1750.032.1418640
CATCTCG1750.032.1418639
AGACCGT1750.032.1418628
CCGAGAC1750.032.1418625
TCGTATG1750.032.1418643
GAGCGGT2050.029.6424036
CGGTTCA2050.029.6320829
GCGGTTC2100.028.9265568
GAGACCG2000.028.1241327
AAGAGCG2300.026.4204034
AGAGCGG2300.026.4204035
ATGCCGA2300.026.41120322
CGGAAGA2400.025.3224951
Produced by FastQC (version 0.11.5)